Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/10910
Blood cell transcriptomic-based early biomarkers of adverse programming effects of gestational calorie restriction and their reversibility by leptin supplementation
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DOI: 10.1038/srep09088
ISSN: 2045-2322
WOS ID: 000351150300001
Scopus EID: 2-s2.0-84924794882
PMID: 25766068
Embase PUI: L612510642
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2015-03-13Document type
research articleCitation
Konieczna J, Sánchez J, Palou March M, Pico C, Palou Oliver A. Blood cell transcriptomic-based early biomarkers of adverse programming effects of gestational calorie restriction and their reversibility by leptin supplementation. Sci Rep. 2015 Mar 13;5:9088.Abstract
The challenge of preventing major chronic diseases requires reliable, early biomarkers. Gestational mild undernutrition in rats is enough to program the offspring to develop later pathologies; the intake of leptin, a breastmilk component, during lactation may reverse these programming effects. We used these models to identify, in peripheral blood mononuclear cells (PBMCs), transcriptomic-based early biomarkers of programmed susceptibility to later disorders, and explored their response to neonatal leptin intake. Microarray analysis was performed in PBMCs from the offspring of control and 20% gestational calorie-restricted dams (CR), and CR-rats supplemented with physiological doses of leptin throughout lactation. Notably, leptin supplementation normalised 218 of the 224 mRNA-levels identified in PBMCs associated to undernutrition during pregnancy. These markers may be useful for early identification and subsequent monitoring of individuals who are at risk of later diseases and would specifically benefit from the intake of appropriate amounts of leptin during lactation.
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https://dx.doi.org/10.1038/srep09088MeSH
Animals, NewbornCluster Analysis
Body Weights and Measures
Gene Expression Regulation
Blood Cells
Leukocytes, Mononuclear
Pregnancy
Transcriptome
Male
Biomarkers
Leptin
Female
Gene Expression Profiling
Rats
Animals
Caloric Restriction
Dietary Supplements
Reproducibility of Results
Genome-Wide Association Study
DeCS
AnimalesReproducibilidad de los Resultados
Biomarcadores
Femenino
Masculino
Restricción Calórica
Regulación de la Expresión Génica
Estudio de Asociación del Genoma Completo
Células Sanguíneas
Ratas
Leucocitos Mononucleares
Embarazo
Animales Recién Nacidos
Suplementos Dietéticos
Leptina
Perfilación de la Expresión Génica
Pesos y Medidas Corporales
Análisis por Conglomerados
Transcriptoma