Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/11361
The Role of Viral and Host MicroRNAs in the Aujeszky's Disease Virus during the Infection Process
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ISSN: 1932-6203
WOS ID: 000330339800072
Scopus EID: 2-s2.0-84899879912
PMID: 24475202
Embase PUI: L373030819
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2014-01-24Document type
research articleCitation
Timoneda O, Nunez-Hernandez F, Balcells I, Muñoz M, Castello A, Vera G, et al. The Role of Viral and Host MicroRNAs in the Aujeszky's Disease Virus during the Infection Process. PLoS One. 2014 Jan 24;9(1):e86965.Abstract
Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky's disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky's disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host - pathogen interaction during viral infection.
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https://dx.doi.org/10.1371/journal.pone.0086965MeSH
Genes, ViralGene Expression Regulation
Cell Line
Olfactory Bulb
Trigeminal Ganglion
Pseudorabies
MicroRNAs
Herpesvirus 1, Suid
Gene Expression Profiling
Gene Regulatory Networks
Animals
Host-Pathogen Interactions
RNA, Messenger
Swine
Virulence
Swine Diseases
DeCS
AnimalesPorcinos
Enfermedades de los Porcinos
Virulencia
Bulbo Olfatorio
Línea Celular
Regulación de la Expresión Génica
Interacciones Huésped-Patógeno
Herpesvirus Suido 1
Seudorrabia
Ganglio del Trigémino
Genes Virales
ARN Mensajero
Redes Reguladoras de Genes
Perfilación de la Expresión Génica
MicroARNs