Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/11909
DNA Methylation Changes are Associated with the Programming of White Adipose Tissue Browning Features by Resveratrol and Nicotinamide Riboside Neonatal Supplementations in Mice
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CitationSerrano A, Asnani-Kishnani Madhu, Couturier C, Astier J, Palou A, Landrier JF, et al. DNA Methylation Changes are Associated with the Programming of White Adipose Tissue Browning Features by Resveratrol and Nicotinamide Riboside Neonatal Supplementations in Mice. Nutrients. 2020 Feb;12(2):461.
Neonatal supplementation with resveratrol (RSV) or nicotinamide riboside (NR) programs in male mice brown adipocyte-like features in white adipose tissue (WAT browning) together with improved metabolism in adulthood. We tested the involvement in this programming of long-term epigenetic changes in two browning-related genes that are overexpressed inWAT of supplemented mice, Slc27a1 and Prdm16. Suckling mice received orally the vehicle, RSV or NR from postnatal days 2-to-20. After weaning (d21) onto a chow diet, male mice were habituated to a normal-fat diet (NFD) starting d75, and split on d90 into continuation on the NFD or switching to a high-fat diet (HFD) until euthanization on d164. CpG methylation by bisulfite-sequencing was analyzed on inguinal WAT. Both treatments modified methylation marks in Slc27a1 and Prdm16 and the HFD-dependent dynamics of these marks in the adultWAT, with distinct and common effects. The treatments also a ffected gene expression of de novo DNA methylases in WAT of young animals (euthanized at d35 in independent experiments). Studies in 3T3-L1 adipocytes indicated the direct effects of RSV and NR on the DNA methylation machinery and favoring browning features. The results support epigenetic effects being involved inWAT programming by neonatal RSV or NR supplementation in male mice.
Animal Nutritional Physiological Phenomena
Adipose Tissue, Brown
Adipose Tissue, White
Tejido Adiposo Pardo
Tejido Adiposo Blanco
Animales Recién Nacidos
Metilación de ADN
Fenómenos Fisiológicos Nutricionales de los Animales