Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/11909
DNA Methylation Changes are Associated with the Programming of White Adipose Tissue Browning Features by Resveratrol and Nicotinamide Riboside Neonatal Supplementations in Mice
Identifiers
DOI: 10.3390/nu12020461
eISSN: 2072-6643
WOS ID: 000522458700188
Scopus EID: 2-s2.0-85079484359
PMID: 32059412
Embase PUI: L2003765660
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2020-02Document type
research articleCitation
Serrano A, Asnani-Kishnani Madhu, Couturier C, Astier J, Palou A, Landrier JF, et al. DNA Methylation Changes are Associated with the Programming of White Adipose Tissue Browning Features by Resveratrol and Nicotinamide Riboside Neonatal Supplementations in Mice. Nutrients. 2020 Feb;12(2):461.Abstract
Neonatal supplementation with resveratrol (RSV) or nicotinamide riboside (NR) programs in male mice brown adipocyte-like features in white adipose tissue (WAT browning) together with improved metabolism in adulthood. We tested the involvement in this programming of long-term epigenetic changes in two browning-related genes that are overexpressed inWAT of supplemented mice, Slc27a1 and Prdm16. Suckling mice received orally the vehicle, RSV or NR from postnatal days 2-to-20. After weaning (d21) onto a chow diet, male mice were habituated to a normal-fat diet (NFD) starting d75, and split on d90 into continuation on the NFD or switching to a high-fat diet (HFD) until euthanization on d164. CpG methylation by bisulfite-sequencing was analyzed on inguinal WAT. Both treatments modified methylation marks in Slc27a1 and Prdm16 and the HFD-dependent dynamics of these marks in the adultWAT, with distinct and common effects. The treatments also a ffected gene expression of de novo DNA methylases in WAT of young animals (euthanized at d35 in independent experiments). Studies in 3T3-L1 adipocytes indicated the direct effects of RSV and NR on the DNA methylation machinery and favoring browning features. The results support epigenetic effects being involved inWAT programming by neonatal RSV or NR supplementation in male mice.
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https://dx.doi.org/10.3390/nu12020461Keywords
DNA methylationmetabolic programming
WAT beiging/browning
food bioactives
B vitamins
dietary polyphenols
MeSH
Animals, NewbornAnimal Nutritional Physiological Phenomena
DNA Methylation
3T3-L1 Cells
Administration, Oral
Resveratrol
Niacinamide
Adipose Tissue, Brown
Epigenesis, Genetic
Male
Animals
Adipose Tissue, White
Dietary Supplements
Mice
DeCS
AnimalesEpigénesis Genética
Células 3T3-L1
Masculino
Administración Oral
Tejido Adiposo Pardo
Tejido Adiposo Blanco
Niacinamida
Animales Recién Nacidos
Metilación de ADN
Resveratrol
Ratones
Suplementos Dietéticos
Fenómenos Fisiológicos Nutricionales de los Animales