Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/12420
Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells
Identifiers
DOI: 10.3390/nu12010146
eISSN: 2072-6643
WOS ID: 000516825500146
Scopus EID: 2-s2.0-85077572916
PMID: 31947975
Embase PUI: L2003459656
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2020-01Document type
research articleCitation
Sureda A, Martorell M, Bibiloni MM, Bouzas C, Gallardo-Alfaro L, Mateos D, et al. Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells. Nutrients. 2020 Jan;12(1):146.Abstract
The aim of this study was to assess free fatty acids' (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids-palmitic, oleic, alpha-linolenic, gamma-linolenic, arachidonic and docosahexaenoic at 50 mu M, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NF kappa B, TLR2, TNF alpha, and COX-2 gene expressions were determined. Palmitic, gamma-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, alpha-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and alpha-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, gamma-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, alpha-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and alpha-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds.
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https://dx.doi.org/10.3390/nu12010146Keywords
PBMCfatty acids
LPS
gene expression
ROS production
MeSH
Metabolic SyndromeAged, 80 and over
Oxidative Stress
Aged
Humans
Leukocytes, Mononuclear
Middle Aged
Hydrogen Peroxide
Male
Fatty Acids, Nonesterified
NF-kappa B
Inflammation Mediators
Tumor Necrosis Factor-alpha
Female
Toll-Like Receptor 2
Interleukin-6
Gene Expression
Lipopolysaccharides
Cyclooxygenase 2
DeCS
LipopolisacáridosExpresión Génica
Interleucina-6
Mediadores de Inflamación
FN-kappa B
Síndrome Metabólico
Femenino
Factor de Necrosis Tumoral alfa
Ácidos Grasos no Esterificados
Masculino
Peróxido de Hidrógeno
Receptor Toll-Like 2
Humanos
Persona de Mediana Edad
Leucocitos Mononucleares
Anciano
Anciano de 80 o más Años
Ciclooxigenasa 2
Estrés Oxidativo