Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/13394
Sperm DNA Integrity and Meiotic Behavior Assessment in an Infertile Male Carrier of a 9qh+++ Polymorphism
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DOI: 10.1155/2011/730847
ISSN: 1110-7243
WOS ID: 000286244300001
Scopus EID: 2-s2.0-79251570856
PMID: 21197455
Embase PUI: L361177915
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2011Document type
research articleCitation
Garcia-Peiro A, Oliver-Bonet M, Navarro J, Abad C, Guitart M, Amengual MJ, et al. Sperm DNA Integrity and Meiotic Behavior Assessment in an Infertile Male Carrier of a 9qh+++ Polymorphism. J Biomed Biotechnol. 2011;:730847. Epub 2010 Dec 9.Abstract
Although several reports on male infertility suggest a relationship between chromosome 9 polymorphisms and infertility, the effects on the phenotype have not been extensively reported. In this study, an infertile patient was found to carry a 9qh+++ chromosome. The flow cytometric TUNEL assay and SCD test have been applied to characterize sperm DNA integrity. In order to assess its meiotic behaviour, synapsis, recombination, and aneuploidy, analyses have been also performed. Sperm DNA fragmentation (SDF) was 77.81% and 87% for the TUNEL and SCD tests, respectively. Ninety-two percent of pachytene cells analyzed showed meiotic abnormalities. The mean number of MLH1 foci per pachytene in the control group was higher (49) than the mean found in the 9qh+++ patient (38) (P < .0001). In spermatozoa, significant increases of disomy rates were observed for chromosome 18 and for the sex chromosomes (P < .0001). These disturbances could be present in other male carriers of a less marked 9qh+.
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https://dx.doi.org/10.1155/2011/730847MeSH
In Situ Nick-End LabelingAdult
Chromosomes, Human, Pair 9
Spermatozoa
DNA
Flow Cytometry
Humans
DNA Damage
Chromatin Assembly and Disassembly
Adaptor Proteins, Signal Transducing
Infertility, Male
Aneuploidy
Nuclear Proteins
Male
Synaptonemal Complex
In Situ Hybridization, Fluorescence
Pachytene Stage
Polymorphism, Genetic
MutL Protein Homolog 1
DeCS
Complejo SinaptonémicoProteínas Adaptadoras Transductoras de Señales
Hibridación Fluorescente in Situ
Proteínas Nucleares
Etiquetado Corte-Fin in Situ
Ensamble y Desensamble de Cromatina
Masculino
Citometría de Flujo
Homólogo 1 de la Proteína MutL
Fase Paquiteno
Aneuploidia
Daño del ADN
Humanos
ADN
Infertilidad Masculina
Cromosomas Humanos Par 9
Adulto
Polimorfismo Genético
Espermatozoides