Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/14191
Expression of Toll-like receptor 2 is up-regulated in monocytes from patients with chronic obstructive pulmonary disease
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eISSN: 1465-993X
WOS ID: 000237396300001
Scopus EID: 2-s2.0-33646508108
PMID: 16606450
Embase PUI: L43705488
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Pons, J; Sauleda, J; Regueiro, V; Santos, C; Lopez, M; Ferrer, J; Agusti, AGN; Bengoechea, JAPublication date
2006-04-10Document type
research articleCitation
Pons J, Sauleda J, Regueiro V, Santos C, Lopez M, Ferrer J, et al. Expression of Toll-like receptor 2 is up-regulated in monocytes from patients with chronic obstructive pulmonary disease. Respir Res. 2006 Apr 10;7:64.Abstract
Background: Chronic obstructive pulmonary disease ( COPD) is characterised by pulmonary and systemic inflammation which flare-up during episodes of acute exacerbation (AECOPD). Given the role of Toll-like receptors (TLRs) in the induction of inflammatory responses we investigated the involvement of TLRs in COPD pathogenesis. Methods: The expression of TLR-2, TLR-4 and CD14 in monocytes was analyzed by flow cytometry. To study the functional responses of these receptors, monocytes were stimulated with peptidoglycan or lipopolysaccharide and the amounts of TNF alpha and IL-6 secreted were determined by ELISA. Results: We found that the expression of TLR-2 was up-regulated in peripheral blood monocytes from COPD patients, either clinically stable or during AECOPD, as compared to never smokers or smokers with normal lung function. Upon stimulation with TLR-2 ligand monocytes from COPD patients secreted increased amounts of cytokines than similarly stimulated monocytes from never smokers and smokers. In contrast, the expressions of TLR-4 and CD14 were not significantly different between groups, and the response to lipopolysaccharide (a TLR-4 ligand) stimulation was not significantly different either. At discharge from hospital TLR-2 expression was down-regulated in peripheral blood monocytes from AECOPD patients. This could be due to the treatment with systemic steroids because, in vitro, steroids down-regulated TLR-2 expression in a dose-dependent manner. Finally, we demonstrated that IL-6, whose plasma levels are elevated in patients, up-regulated in vitro TLR-2 expression in monocytes from never smokers. Conclusion: Our results reveal abnormalities in TLRs expression in COPD patients and highlight its potential relationship with systemic inflammation in these patients.
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https://dx.doi.org/10.1186/1465-9921-7-64MeSH
AgedMonocytes
Toll-Like Receptor 2
Pulmonary Disease, Chronic Obstructive
Gene Expression Regulation
Humans
Cells, Cultured
Up-Regulation
Middle Aged
DeCS
Regulación de la Expresión GénicaEnfermedad Pulmonar Obstructiva Crónica
Receptor Toll-Like 2
Humanos
Persona de Mediana Edad
Células Cultivadas
Regulación hacia Arriba
Anciano
Monocitos