Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/14506
gamma delta T lymphocytes from cystic fibrosis patients and healthy donors are high TNF-alpha and IFN-gamma-producers in response to Pseudomonas aeruginosa
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ISSN: 1465-993X
WOS ID: 000185781800001
Scopus EID: 2-s2.0-25444479344
PMID: 14525626
Embase PUI: L41358100
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2003-09-08Document type
research articleCitation
Raga S, Julia M, Crespi C, Figuerola J, Martinez N, Mila J, et al. gamma delta T lymphocytes from cystic fibrosis patients and healthy donors are high TNF-alpha and IFN-gamma-producers in response to Pseudomonas aeruginosa. Respir Res. 2003 Sep 08;4(9):9. Epub 2003 Sep 8.Abstract
Background: gammadelta T cells have an important immunoregulatory and effector function through cytokine release. They are involved in the responses to Gram-negative bacterium and in protection of lung epithelium integrity. On the other hand, they have been implicated in airway inflammation. Methods: The aim of the present work was to study intracytoplasmic IL-2, IL-4, IFN-gamma and TNF-alpha production by gammadelta and alphabeta T lymphocytes from cystic fibrosis patients and healthy donors in response to Pseudomonas aeruginosa (PA). Flow cytometric detection was performed after peripheral blood mononuclear cells (PBMC) culture with a cytosolic extract from PA and restimulation with phorbol ester plus ionomycine. Proliferative responses, activation markers and receptor usage of gammadelta T cells were also evaluated. Results: The highest production of cytokine was of TNF-alpha and IFN-gamma, gammadelta being better producers than alphabeta. No differences were found between patients and controls. The Vgamma9delta2 subset of gammadelta T cells was preferentially expanded. CD25 and CD45RO expression by the alphabeta T subset and PBMC proliferative response to PA were defective in cystic fibrosis lymphocytes. Conclusion: Our results support the hypothesis that gammadelta T lymphocytes play an important role in the immune response to PA and in the chronic inflammatory lung reaction in cystic fibrosis patients. They do not confirm the involvement of a supressed Th1 cytokine response in the pathogenesis of this disease.
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https://dx.doi.org/10.1186/1465-9921-4-9MeSH
ChildReceptors, Antigen, T-Cell, gamma-delta
Receptors, Antigen, T-Cell, alpha-beta
Adult
Flow Cytometry
Humans
Cystic Fibrosis
Ionomycin
Phenotype
Male
Tetradecanoylphorbol Acetate
Cytokines
Monocytes
Tumor Necrosis Factor-alpha
Female
Receptors, Interleukin-2
Pseudomonas Infections
Leukocyte Common Antigens
Case-Control Studies
Intracellular Membranes
Kinetics
Adolescent
Cells, Cultured
Cell Proliferation
T-Lymphocytes
Interferon-gamma
DeCS
Linfocitos TFemenino
Monocitos
Adolescente
Masculino
Células Cultivadas
Membranas Intracelulares
Cinética
Receptores de Antígenos de Linfocitos T gamma-delta
Receptores de Antígenos de Linfocitos T alfa-beta
Receptores de Interleucina-2
Proliferación Celular
Citocinas
Acetato de Tetradecanoilforbol
Ionomicina
Interferón gamma
Factor de Necrosis Tumoral alfa
Citometría de Flujo
Fibrosis Quística
Humanos
Fenotipo
Antígenos Comunes de Leucocito
Niño
Infecciones por Pseudomonas
Adulto
Estudios de Casos y Controles