Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/16297
Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients
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ISSN: 1471-2466
WOS ID: 000472880900001
Scopus EID: 2-s2.0-85068169882
PMID: 31234826
Embase PUI: L628244679
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Millares, Laura; Pascual, Sergi; Monton, Concepcion; Garcia-Nunez, Marian; Lalmolda, Cristina; Faner, Rosa; Casadevall, Carme; Seto, Laia; Capilla, Silvia; Moreno, Amalia; Angelica Castro-Acosta, Ady; Jose Alvarez-Martinez, Carlos; Sibila, Oriol; Peces-Barba, German; Cosio, Borja G

Publication date
2019-06-24Document type
research articleCitation
Millares L, Pascual S, Monton C, Garcia-Nunez M, Lalmolda C, Faner R, et al. Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients. BMC Pulm Med. 2019 Jun 24;19:112.Abstract
BackgroundThe respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear.MethodsCross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME.ResultsWe observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with 2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p=0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels 2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p=0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p=0.020], and a significant increase in the RAs of 20 genera.ConclusionThe respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels.
Publisher version
https://dx.doi.org/10.1186/s12890-019-0867-xMeSH
Disease ProgressionEosinophils
Aged
Sputum
Leukocyte Count
Pulmonary Disease, Chronic Obstructive
Humans
Forced Expiratory Volume
Middle Aged
Cross-Sectional Studies
Lung
Microbiota
Male
Multivariate Analysis
Respiratory System
Severity of Illness Index
Prospective Studies
RNA, Ribosomal, 16S
Female
DeCS
MicrobiotaFemenino
Análisis Multivariante
Pulmón
Masculino
Estudios Transversales
Volumen Espiratorio Forzado
Enfermedad Pulmonar Obstructiva Crónica
Humanos
Persona de Mediana Edad
Estudios Prospectivos
ARN Ribosómico 16S
Anciano
Sistema Respiratorio
Índice de Severidad de la Enfermedad
Progresión de la Enfermedad
Eosinófilos
Recuento de Leucocitos
Esputo
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Hospital Universitario Son Espases - HUSE > Comunicación científicaInstituto de Investigación Sanitaria Islas Baleares - IDISBA > Comunicación científica