Identifier to cite or link to this item: https://hdl.handle.net/20.500.13003/19087
Challenges in the diagnosis of hypervitaminemia B12. Interference by immunocomplexes
StatisticsItem usage statistics
MetadataShow Dublin Core item record
Document typeresearch article
CitationDelgado JA, Pastor García MI, Jiménez NM, Petit GC, Pablo Cànaves JA, Robles J, et al. Challenges in the diagnosis of hypervitaminemia B12. Interference by immunocomplexes. Clin Chim Acta. 2023 Feb;541:117267.
High vitamin B12 concentrations are considered a common finding in clinical practice. Thanks to immunoassay accessibility, vitamin B12 has become a usual test in routine health checkups. However, these analytical methods usually present antibody-mediated interferences. Our aim was to propose an algorithm for the screening of antibody-mediated analytical interferences on vitamin B12 immunoassays on the Alinity platform. Observational, prospective, case-control study was performed during 12 months. Individuals with persistently elevated cobalamin concentrations [>554 pmol/L] were considered as cases in the absence of supplementation or other justifying cause. Individuals under treatment with vitamin B12, or in the context of alcoholism were included as controls. A thorough interference study by macromolecules in immunoassays was performed in serum samples: PEG precipitation, rheumatoid factor, heterophile antibodies and gel permeation chromatography (GPC). Albumin, total B12, IgG and IgM were measured in every GPC collected fraction and chromatograms were drafted. Up to 45% of cases presented interference by B12-immunocomplexes and the precipitation for all of them was >50%. The individual with the lowest interfered vitamin B12 result was 661 pmol/L. The presence of antibody-mediated interferences, mainly B12-immunocomplexes, is a relatively common phenomenon. A simple algorithm for the screening of interferences is useful and reliable in ruling out healthy individuals and highly cost-effective.
Vitamin B 12 Deficiency
Vitamin B 12
Vitamina B 12
Deficiencia de Vitamina B 12
Estudios de Casos y Controles
This item appears in following Docusalut collectionsHospital Universitario Son Espases - HUSE > Comunicación científica
Instituto de Investigación Sanitaria Islas Baleares - IDISBA > Comunicación científica