Identifier to cite or link to this item: http://hdl.handle.net/20.500.13003/9740
A novel pharmacodynamic assay to evaluate the effects of crystallization inhibitors on calcium phosphate crystallization in human plasma
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Document typeresearch article
CitationFerrer Reynés MD, Perez MM, Canaves MM, Buades-Reine'S JM, Salcedo C, Perello Bestard J. A novel pharmacodynamic assay to evaluate the effects of crystallization inhibitors on calcium phosphate crystallization in human plasma. Sci Rep. 2017 Jul 31;7:68581.
Cardiovascular calcification (CVC) is a progressive complication of chronic kidney disease and a predictor of CV events and mortality. The use of bionarkers to predict CV risk and activities of potential or current treatment drugs in these patients could have a crucial impact on therapeutic approaches. Our aim was to develop a novel assay for measurement of the rate of calcium phosphate crystallization in human plasma and provide a tool to evaluate the effects of crystallization inhibitors. The efficacy of inhibitors was determined by adding inhibitory compounds (polyphosphates, fetuin-A, sodium thiosulfate or citrate) to control samples. The assay was additionally validated for SNF472, an experimental formulation of phytate being developed for the treatment of calciphylaxis and CVC in patients with end-stage renal disease (ESRD) undergoing hemodialysis (HD). The method was repeatable and reproducible. The plasma crystallization rate was reduced up to 80% in a concentration-dependent manner following treatment with inhibitors in vitro, among which SNF472 was the most potent. This method appears beneficial in evaluating and discriminating between inhibitory activities of compounds such as polyphosphates on calcium phosphate crystallization, which present a novel therapeutic approach to treat CVC in ESRD patients.
MeSHCalcium Chelating Agents
Drug Evaluation, Preclinical
Evaluación Preclínica de Medicamentos
Quelantes del Calcio
Fosfatos de Calcio
This item appears in following Docusalut collectionsInstituto de Investigación Sanitaria Islas Baleares - IDISBA > Comunicación científica
Hospital Universitario Son Llàtzer - HUSLL > Comunicación científica