Fuster Aparisi, AlbertoEscarrer-Garau, GabrielTruyols-Vives, JoanGarcia Moll, LluciaCañete Cánaves, MarcEstrany Celià, Maria del MarRibas Taberner, Maria Del MarQuetglas-Llabrés, Maria MagdalenaGonzález López, MartaAlayón Afonso, RafaelMonserrat Mesquida, MargalidaTejada, SilviaFerrer Reynés, Miguel DavidSureda, AntoniMiró Lladó, ManelMercader-Barceló, JosepFuster Aparisi, Alberto2024-09-132024-09-132024Fuster Aparisi A, Escarrer Garau G, Truyols Vives J, Garcia Moll L, Cañete Cánaves M, Estrany Celià MM, Ribas MM, Quetglas Llabrés MM, González López M, Jiménez García M, Alayón Afonso R, Monserrat Mesquida M, Tejada Gavela S, Ferrer Reynés MD, Sureda Gomila A, Miró Lladó M, Mercader Barceló J. Bioavailability and organ-specific impacts of polyethylene-adsorbed bisphenol A compared to free bisphenol A in rats. Poster presented at XLII Reunión anual de la Sociedad Española de Epidemiología (SEE); 10-13 de september 2024; Cádiz.https://hdl.handle.net/20.500.13003/20994Introduction: Microplastics (MP) are emerging contaminants ubiquitously present in the food chain. Increasing evidence indicates that MP can be absorbed and accumulated in organs. Plastic additives of MP raise as a major health concern. Objective: To analyze the bioavailability and health impact of polyethylene (PE)-adsorbed bisphenol A (BPA-PE) and free BPA. Experimental design: In the study I, rats were gavaged with 2 mg/kg free BPA, BPA-PE (0.67 g PE/kg), or their respective vehicles. In the study II, rats were gavaged with 0,67 mg/kg free BPA, BPA-PE (0.22 g PE/kg), pristine PE, or the combined vehicles. All rats were sacrificed 24 h after administration. Results: Plasma glucuronidated BPA (gBPA) was detected in BPA-PE rats of both studies. In the study I, only free BPA administration induced (P<0.05) the activity of hepatic antioxidant proteins (GPx, SOD). Conversely, only BPA-PE administration altered (P<0.05) the activity of lung antioxidant enzymes (GRd, SOD). Moreover, only BPA-PE increased the expression of genes involved in oxidative stress (Mn- Sod, iNos) in the liver, adipose tissue (AT), and lung; in lung repair (Tgfb, Col1a1); and in AT lipogenesis (Srepb1c, leptin). In the study II, gBPA levels from BPA-PE group were not different from those found in the free BPA group. In the gut, both free BPA and BPA-PE increased (P<0.05) the activity of a prooxidant enzyme (MPO), a phase II detoxification enzyme (GST), and the expression of Mrd1, involved in the protection from toxics. Both pristine PE and BPA-PE induced (P<0.05) Tgfb mRNA levels in the lung. Conclusions: The bioavailability of MP-adsorbed BPA is similar to that of free BPA. However, the body distribution of BPA-PE appears to differ from that of free BPA, affecting organs such as the lung.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/conference posteropen accessBisphenol AMicroplasticsBioavailabilityOxidative StressTissue Repair